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IAM星火大讲堂第10期:美国西北大学Milan Mrksich教授做IAM客星火大讲堂
阅读次数:661 添加时间:2016/11/27 发布: 管理员



报 告 人:Milan Mrksich教授
报告题目:Combining Arrays and Mass Spectrometry for High Throughput Discovery in Chemistry and Biology


Short Bio: 
Milan Mrksich is the Henry Wade Rogers Professor at Northwestern University, with appointments in the Departments of Biomedical Engineering, Chemistry, and Cell and Molecular Biology.  He also serves as the Founding Director of the Center for Synthetic Biology and as an Associate Director of the Robert H. Lurie Comprehensive Cancer Center.  He earned a BS degree in Chemistry from the University of Illinois and a PhD in Chemistry from Caltech.  He then served as an American Cancer Society Postdoctoral Fellow at Harvard University before joining the faculty at the University of Chicago in 1996.  He began his current position at Northwestern in 2011.  His many honors include the Camille Dreyfus Teacher-Scholar Award, the TR100 Innovator Award, Arthur C. Cope Scholar Award, election to the American Institute for Medical and Biological Engineering and the Illinois Bio ICON Innovator Awardee.  Professor Mrksich is an active advisor in government and industry.  His present and past appointments include the Chair of the Defense Sciences Research Council—an advisory group to the Defense Department—a member of the Board of Governors for Argonne National Laboratory, and Chair of the Searle Scholars Advisory Board. 


This talk will describe an approach for using mass spectrometry to analyze molecular arrays.  The arrays are prepared by immobilizing small molecules, proteins, peptides and carbohydrates to self-assembled monolayers of alkanethiolates on gold.  This arrays are then treated with reactants—either chemical reagents or enzymes—and then analyzed using the SAMDI technique to identify the masses of substituted alkanethiolates in the monolayer and therefore a broad range of reactivities and post-translational modifications—including kinase, protease, methyltransferase and carbohydrate-directed modifications—and for discovering chemical reactions.  This talk will describe applications to high throughput experiments, including the discovery of reactions, the use of carbohydrate arrays to discover novel enzymes, the preparation of peptide arrays to profile the enzyme activities in cell lysates and high-throughput screening to discover novel reactions and small molecular modulators.  These examples illustrate the broad capability of the SAMDI method to profile and discover molecular activities in the molecular sciences.


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